|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. The CNS of homozygous H99 embryos is not grossly deformed.
(A,B). Axon tracts are visualized using the BP102 antibody in wt
(A) and H99 (B) embryos. Their pattern is similar, although thickened
junctions between the longitudinal connectives and the commissures (arrow),
and a widened midline (bracket) are visible in H99. Note that the CNS
of H99 embryos is generally wider than that of wt due to additional
cells. (C,D) FasII staining of axons reveals a variably altered
pattern. The H99 embryo shown in D has a more extreme phenotype in
that the fascicles are somewhat disordered and fuzzy. (E,F)
Glial cells, visualized with the anti-Repo antibody. A stack of scans was made
throughout the CNS and the scans then projected together to show all
Repo-positive cells. The positioning of glia is only slightly affected in
H99. All images show four abdominal segments (A3 to A6) of late stage
16 embryos; anterior is up.
|
