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Fig. 5. DiI-labeled clones of NB1-1a and NB7-3 in H99 embryos. Each
plate shows a semischematic illustration of a wt and an H99 clone,
and the corresponding image of the H99 clone. Glia are depicted in
green, motoneurons in red. Dashed lines indicate the CNS midline. (A-C)
No difference can be seen between NB1-1a in wt (A) and H99 (B,C).
Shown is an H99 clone containing 11 cells (including three glial
cells). Both the cell numbers and the axonal projection pattern are unchanged
in H99. (D-F) NB7-3 consists of more cells in H99
(E,F) than in wt (D), including an additional motoneuron that projects its
axon outside the CNS (arrow). The image shows an abdominal H99 clone
with 9 cells.