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Fig. 6. DiI-labeled clones of NB4-2, NB5-3, NB7-2 and NB7-4. Each plate shows an illustration of a wt and an H99 clone, and the corresponding image of the H99 clone. Glia are depicted in green, motoneurons in red. Dashed lines indicate the CNS midline. (A-C) The NB4-2 lineage has two motoneuronal projections in wt (A). In H99, there is a third motoneuronal axon (arrow in C) projecting ipsilaterally through the posterior root of the intersegmental nerve. The image shows an abdominal clone comprising 25 cells. (D-F) The wt NB5-3 shows an ipsilateral motoneuronal and two contralateral interneuronal projections (D). In H99 (E,F), there is an additional ipsilateral interneuronal projection extending anteriorly (arrow in F). Shown is an image of an abdominal clone containing 25 cells. (G-I) In the wt, NB7-2 interneurons form two fascicles, an ipsilateral one extending posteriorly, and a contralateral one projecting through the posterior commissure (G). The H99 clones (H,I) show another contralateral projection, extending alongside the wt one (arrow in I). Shown is an abdominal H99 clone with 21 cells. (J-L) In the wt, NB7-4 interneurons form one fascicle that traverses contralaterally through the posterior commissure (J). In H99 (K,L), NB7-4 clones exhibit an atypical projection that runs through the anterior commissure of the next segment (arrow in L). Shown is an abdominal clone with 29 cells.

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