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Fig. 4. Expression of Ccn2 and follistatin is induced by Eda-A1.
Eda-deficient (A,B) or wild-type (C) E14
individual skin explants were cut in two halves along the midline and cultured
in the absence or presence of 2 µg/ml Eda-A1, and gene expression was
analysed by quantitative RT-PCR. (A) A timecourse of expression of Ccn2,
Smad7, follistatin and I-
B
in
Eda-deficient skin upon Eda-A1 stimulus. Upregulation of
Ccn2 is detected already after 1 hour of Eda treatment and peaks at
3-4 hours. I-B is induced with kinetics
similar to Ccn2. (B) Comparison of Ccn2 induction by Eda-A1
in skin explants cultured in a medium with or without serum. Ccn2
transcripts are maintained at high level for a longer time in the absence of
serum. (C) Induction of Ccn2, Smad7 and follistatin gene expression
in wild-type skin after exposure to Eda-A1 in the absence of serum.
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