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Fig. 5. Edar and Ccn2 colocalize during early hair and tooth
development. Whole-mount in situ hybridization with a probe specific to
Ccn2 (A-G,H,K,M) and Edar
(I,J,L). (A-C') Ccn2 is expressed in
primary hair placodes of wild-type embryos at E14, and is often concentrated
at the circumference of the placode (arrowheads in C). (D) No localized
expression of Ccn2 is detected in Eda-null skin at E14.
(E-G) Recombinant Eda-A1-induced localized upregulation of Ccn2 in
Eda-/- E13 skin explants cultured for 24 hours. (H,I)
Vibratome section of whole mounts confirmed the colocalization of
Edar and Ccn2 in primary hair placodes of E14 wild-type
embryos. (J,K) Edar and Ccn2 are coexpressed also in
wild-type molar (arrow) and incisor (arrowhead) placodes at E12. (L,M) In
contrast to primary hair placodes, expression of Ccn2 is unaffected
in Eda-deficient tooth placodes. Mc, Meckel's cartilage. Scale bar: 1
mm in A; 0.5 mm in B-D,J-M; 50 µm in H,I.
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