DEV02705 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Role of fezl in forebrain patterning. The expression of foxg1 (A) and six3b (B). The expression of fezl (purple) is complementary to the eye field marker rx3 (red, C-D). (E-F) The expression of fezl (red) and six3b (purple). (G-H) The expression of fezl (G) and six3b (H).

• Supplemental Figure 2 -

  Fig. S2. Phenotypes of early stage fezl morphants. (A-F) Bud stage showing largely normal expression of irx3a, tlc1 and wnt1. (G-J) Mid-somitogenesis stage showing largely normal expression of wnt1 and wnt8b.

• Supplemental Figure 3 -

  Fig. S3. Forced expression of fezl by heat shock. The expression of fezl reached a maximum level approximately 2 hours after heat shock (A) and was maintained for another 2 hours (B). 6 hours later, ectopic fezl expression was still detectable in the double transgenic embryos (C).