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Fig. 4. Overexpression of fezl results in an expansion of the
prethalamus and the elimination of the ZLI in a dose-dependent fashion.
The hsp-gal4 transgenic fish are crossed with uas-fezl
transgenic fish and embryos are heat shocked at 37°C for 15-45 minutes at
75% epiboly. Embryos are stained with indicated probes and analyzed at 28
hpf. Genotypes are identified by PCR with DNA from the tail. (A-D) The
expression of dlx2a in the prethalamus is mostdramatically expanded.
Its expression in the ventral telencephalon is mildly increased whereas its
expression in the pharyngeal arches (pa) is not significantly altered. Arrow
in A-D indicates dlx2a-expressing hypothalamic cells; black arrowheads in A-D
indicate the pharyngeal arches; gray arrowhead in B-D indicates ectopic
dlx2a-expressing cells in the midbrain. (E-H) The ZLI, as shown with
shh expression, is moved caudally or completely eliminated depending
on the duration of heat shock, but shh expression in the floor plate
(fp) is unaffected. Square brackets indicate the ZLI. All are lateral views.
hy, hypothalamus; pt, prethalamus; tel, telencephalon; fp, floor plate; bp,
basal plate; pa, pharyngeal arches.
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