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Figure 6


Fig. 6. SmB-binding domain in Csul. (Upper panel) Full-length GST-Csul or derivatives were purified from bacteria and incubated with [35S]S*Tag-SmB. Bound [35S]S*Tag-SmB proteins were separated by SDS-PAGE and detected by fluorography. Amino acid numbers are given across the top. `Input' was one tenth of the synthesized [35S]S*Tag-SmB proteins. The smallest fragment of Csul showing binding to SmB encompasses amino acids 1-200. (Lower panel) The amount of GST-Csul polypeptides was visualized by Coomassie Blue staining. (Right) Representation of the GST-Csul fragments used for the mapping and the summary of the results, with the identified domain required for SmB-binding in Csul shown in blue.





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