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Figure 1


Fig. 1. Drosophila Na,K-ATPase ß-subunits have distinct subcellular localizations. (A-C) Nrv1 (red) is expressed in many epithelial tissues, including the epidermis (A1,2), salivary gland (at low levels; B1,2), and trachea (C1,2). Nrv1 localizes to regions basal to and distinct from the SJ as marked by Nrv2 (green; A1,B1,C1). No Nrv1 protein is detectable in the trans-heterozygote of two small deficiencies [Df(ED)7007 and DF(ED)6569] that delete both nrv1 and nrv2 (A3,B3,C3). Levels of Nrv1 staining are unaffected by a nrv2-null mutation but the domain of Nrv1 localization is extended apically into the region normally occupied by SJs (A4,B4,C4). (D) Nrv3 is expressed in the chordotonal organ (D1) and the central nervous system (D2). In A1,B1,C1, the apical surface is denoted by dashed blue lines and the basal surface by dashed white lines. Scale bars: in A1, 5 µm for A1-B4,D1; in C1, 5 µm for C1-C4; in D2, 5 µm.





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