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Figure 4


Fig. 4. Rat {alpha}1 and some Drosophila ATP{alpha} isoforms have SJ full activity. (A,B) The Na,K-ATPase {alpha}-subunit (A) is encoded by one major locus at 93A that generates numerous isoforms by alternative splicing (B). Splice forms differ in the N-terminal 39 aa (green) and in a mutually exclusive exon (blue). The catalytic residue D369 (D394 in the fly) is denoted by a red dot. (C-H) Compared with the WT (C1-5), Atp{alpha} [DTS1R2] mutants have long DTs with diameter defects (D1) and missing lumens in the GBs (D2). The mutant fails to exclude dye from the trachea (D3), has disorganized SJs (D4; Coracle, green; ATP{alpha}, red), and no longer accumulates Verm in the tracheal lumen [D5 and Wang et al. (Wang et al., 2006)]. DT and GB morphology, barrier junction, Verm accumulation defects and Coracle localization are fully rescued when Long C (F1,4,5), Long C D->N (G1,4,5), or rat {alpha}1 (H1,4,5) are expressed using a da-Gal4 driver. Expression of Short C gives only slight DT rescue (E1), no Coracle rescue (E4) and no Verm rescue (E5). Scale bars: in H2, 10 µm for C-H images 1,2; in H3,10 µm for C-H image 3; in H5, 5 µm for C-H images 4,5.





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