Supplemental Figure 1
-
Fig. S1. Genomic rescue fragments used to identify the egg gene. Shown is a restriction map of the 9 kb GC64N DNA fragment, including the positions of restriction sites used to make the NΔ1-NΔ4 constructs. The positions of DNA segments that encode 3.9, 1.1 and 1.4 kb mRNAs are indicated above the map. The righthand column indicates whether a DNA fragment rescued the female-sterility of egg mutations. The genomic region identified by NΔ2 and NΔ4 contains two annotated genes predicted by the BDGP, CG30422 and CG30426. Our analysis has shown that both are part of one transcription unit that encodes the HMT described in this report. B, BamH1; Bg, BglII; E, EcoRI; K, KpnI; N, NdeI; Nh, NheI; Xb, XbaI.
