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Fig. 1. The Drosophila eggless (egg) gene encodes a SET domain protein similar to human/mouse SETDB1/ESET. (A) Northern blot of ovary poly(A+) RNA hybridized with a probe made from the 5' end of the egg gene. A 3' probe labels the same 3.9 kb band. (B) Western blot of ovary extracts, labeled with an antibody to Egg. Two major protein bands, 170 and 140 kDa, are detected in wild-type ovaries. These proteins are strongly reduced in egg²³⁵ ovaries, and replaced with a smaller 59 kDa protein. (C) Proposed structure of the egg transcript. This structure is based on the assembly of the complete sequences of three overlapping ovary cDNAs and two embryonic cDNAs. (D)The predicted protein structures of Egg and SETDB1, showing the identified protein domains within each protein. (E) The egg²³⁵ allele contains two nucleotide substitutions near the 5' end of intron 4 (shown in large bold letters). Failure to splice out this intron introduces a premature stop codon (shown in uppercase letters). (F) The predicted protein product of the egg¹⁴⁷³ allele. This allele contains an 856 bp in-frame deletion that removes the entire SET domain. WT, wild type.

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