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Figure 4


Fig. 4. Patterns of Histone H3 Lysine 9 (H3K9) methylation during oogenesis. Wild-type ovaries labeled with antibodies to dimethyl-H3K9 (H3K9me2) or trimethyl-H3K9 (H3K9me3), and an antibody to Vasa, which labels germ cells, or a DNA dye. Left panels, methylated H3K9 alone; right panels, merged images of H3K9me2 or H3K9me3 (green) and Vasa (red) or DNA (red). (A) H3K9me2 is present in both the germ cells and somatic cells in the germarium and newly budded egg chambers (upper panels). As oogenesis proceeds (stage 4 and 6 egg chambers in bottom panels), the H3K9me2 signal increases in the somatic cells relative to the germ cells. A nurse cell nucleus, germ stem cell nucleus (arrowhead) and follicle cell nuclei (arrows) are indicated. (B) In the germarium (top panels) H3K9me3 is present in both the germ cells and somatic cells, with higher levels apparent in the germ cells. A germ stem cell (arrowhead) and somatic cells (arrow) are indicated. In later stages (stage 4 and 6 egg chambers in bottom panels), H3K9me3 persists in the germ cells but is reduced in somatic cells. The signal is relatively weak in nurse cell nuclei compared with the oocyte nucleus (*). G, germarium; NC, nurse cell nuclei; S2/S4/S6, stage 2/4/6 egg chambers.





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