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Fig. 6. Staining for bone and cartilage reveals abnormalities of the otic capsule and ossicles in mature and developing small Dusp6-deficient animals. (A,B) Lateral views of left temporal bones of control (A) and small heterozygous hearing-impaired (B) P22 animals. The shape of the opening to the middle ear cavity is emphasized with a black line, and an abnormal notch in the heterozygote is indicated with an arrow. (C,D) Lateral views of left ears of P0 control (C) and small heterozygous (D) P0 animals are shown with some of the cochlea dissected away to enable better visualization of the ossicles. The malleus and stapes and incus are indicated. (E,F) Lateral views of right inner ears of P5 wild-type (E) and small Dusp6^L/L (F) pups. Black arrow in E indicates a normal dorsal otic capsule, and gray arrow in F indicates failure of the corresponding region of the mutant otic capsule to form Alcian Blue-positive cartilage. (G,H) Right ossicles dissected from P5 wild-type (G) and small Dusp6^L/L (H) pups. All three mutant ossicles have regions in which cartilage (blue) is missing or weakly staining. (I,J) Lateral views of left temporal bones from wild-type (I) and small, Dusp6^L/L (J) P10 pups. Black arrow in I indicates normal development of the dorsal otic capsule, whereas gray arrow in J indicates that the corresponding region of the mutant capsule has failed to form Alizarin Red-positive bone. (K,L) Left middle ears from wild-type (K) and small Dusp6^L/L (L) P10 pups. The mutant malleus is malformed. i, incus; m, malleus; s, stapes.

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