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Figure 8


Fig. 8. Gata6 and Nkx2.1 interact at specific regions within the upstream regulatory regions of Scd1 and Dpp4. (A) mVista analysis was used to determine conserved regions in the upstream regulatory sequences of Scd1 and Dpp4. Regions probed using ChIP analysis are highlighted in brackets. Red underlined regions are genomic enhancers used in transactivation reporter assays. (B) ChIP analysis demonstrates an interaction of Gata6 with regions A and B in Scd1 and region A in Dpp4, whereas Nkx2.1 interacts with regions A and B in Scd1 and regions A and C in Dpp4. (C) Q-PCR of ChIP reactions on the same regions shown in A and B demonstrating quantitative differences in binding of Gata6 and Nkx2.1 to the identified conserved regions in the Scd1 and Dpp4 regulatory regions. *P<0.01 Gata6 and Nkx2.1 antibodies versus normal IgG.





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