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Figure 3


Fig. 3. fozi-1 encodes a novel protein. (A) fozi-1 gene structure (not drawn to scale) showing molecular lesions of fozi-1 mutants. The first intron of fozi-1 spans 7 kb upstream of the translation start site. (B) The structural motifs of FOZI-1 include a Q-rich region (amino acids 88-133), two C2H2 zinc finger motifs (amino acids 177-201) and an FH2 formin homology domain (amino acids 369-732). (C) pNMA04, pNMA27 and pNMA28 are fozi-1 fusion constructs used to purify recombinant FOZI-1 fragments. pNMA36 and pNMA37 were used in transgenic assays.





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