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Fig. 4. Expression pattern of FOZI-1 in wild-type, cc609 and
cc610 hermaphrodites and embryos. All images are lateral views
with anterior to the left and dorsal up (unless otherwise noted). (A-D)
Wild-type (A,B) and cc609 (C,D) embryos stained with anti-FOZI-1
antibody (A,C) and DAPI (B,D). Nuclear FOZI-1 is seen in a subset of cells at
the 2-fold stage of embryogenesis in wild-type (A) but not in cc609
(C) embryos. (E,F) FOZI-1 staining in a wild-type L1 larva on
two different focal planes. FOZI-1 was observed in the nuclei of 7-12 head
neurons (arrow, E) and 5-7 cells along the ventral nerve cord (arrowheads,
E,F) and in cells derived from the M lineage (red box, E,F). (G-R)
Double labeling with anti-FOZI-1 antibody and hlh-8::gfp at the 2-M
(G-I), 16-M (J-L), 18-M (M-O) and 2-SM (P-R) stages in wild-type animals.
Anti-FOZI-1 antibody staining is shown in panels G,J,M,P; hlh-8::gfp
is shown in panels H, K,N,Q; the corresponding merged images are shown in
panels I,L,O,R. FOZI-1 staining was first detected at the 2-M stage (G-I) and
persisted through the 16-M (J-L) stages. At the 18-M stage (M-O) FOZI-1 is
still present in all undifferentiated CC and BWM precursors but is absent in
the SMs. Arrowheads in panel M denote cells in the ventral nerve cord. (P-R)
FOZI-1 is not present in the 2-SM cells and the subsequent SM lineage (data
not shown). (S) Summary of FOZI-1 expression in the M lineage. The
wild-type M lineage is shown with overlay of FOZI-1 expression highlighted in
red. (T,U) Staining of cc609 (T) and cc610 (U)
animals at mid-L1 stage using anti-FOZI-1 antibodies. (T) No FOZI-1 was
detected in cc609 animals. (U) Wild-type level and localization
pattern of FOZI-1 are detected in cc610 mutant animals in head
neurons (arrow), ventral nerve cord (arrowheads) and the M lineage (red
box).
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