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Fig. 1. Expression of Foxo3a in WT mouse oocytes. (A-D)
Immunostaining of Foxo3a in ovarian follicles of postnatal mice. Ovaries from
8-day-old mice were collected and embedded in paraffin, and sections were used
for staining of Foxo3a and then counter-stained with Hematoxylin. The
immunohistochemistry signals appear as a reddish-brown color and
counter-stained background appears as a blue color. (B,C) Images at a higher
magnification. (D) Negative control for immunostaining of Foxo3a, in which
primary antibody against Foxo3a was not included. (E) Expression of
Foxo3a in oocytes that were larger or smaller than 25 µm. Oocytes were
isolated from 17- to 20-day-old female C57/BL6 mice and sorted as described in
Materials and methods. Levels of ß-actin are shown as an
internal control. All experiments were repeated at least three times, and
representative results are shown; for each experiment, n=15-20.
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