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Fig. 4. Arrested granulosa cell proliferation and reduced FSH receptor
expression in Zp3-Foxo3a Tg mice. Twenty-five-day-old Tg or WT mice were
used for ovarian staining of PCNA and FSH receptor, or for the BrdU
incorporation assay. (A,B) Immunostaining of PCNA expression in
ovarian sections from WT (A, arrow) and Tg (B, arrow) mice. Signals appear in
brown using DAB as substrate. (C,D) Reduced incorporation of
BrdU into granulosa cells of Tg mice (D, arrow) as compared with granulosa
cells of WT littermates (C, arrow). Signals appear reddish-brown using AEC as
substrate. (E,F) Reduced expression of FSH receptor mRNA in
ovaries of Tg mice (F, arrows) as compared with that in ovaries of WT mice (E,
arrows), as measured by in situ hybridization. Signals appear in black.
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