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Fig. 2. GeneChips generated from laser-captured cells parallel earlier studies
but additionally identify numerous transcription factor mRNAs specifically
enriched in ZCs. (A) Dendrogram showing how gene expression
profiles generated from laser-capture microdissected cells for the current
study cluster with previous profiles of the same cell types. Numbers at
branches are modified Pearson's correlations: where 0 means that profiles are
identical, 1 means that they are wholly unrelated and 2 means that they are
inversely related. Correlations were calculated by comparing the fractional
representation of each GO term in each expression profile using GOurmet
software (Doherty et al.,
2006). LCM, laser-capture microdissected profiles in the current
study; Mills et al., ZCs purified by digesting stomachs followed by
centrifugation purification (Mills et al.,
2003); Mueller et al., data from another previous study
(Mueller et al., 2004).
(B) Individual GO terms that differentiate each cell population have
been selected to show how the types of genes enriched in each expression
profile match known functions associated with those cell lineages. Genes
described by the GO term `extracellular space' are highly represented in
profiles of ZCs with a function of secretion; those described by the term
`mitochondrion' are enriched in parietal cells, which are characterized by
abundant mitochondria; those described as `actin-binding' are most common in
the rapidly migrating, cytoskeleton-fortified pit cells. (C) The same
data as in A have been rendered to show the Pearson's correlation between the
current ZC profile and all others. Note that the current ZC is most closely
related to the previous ZC profile. (D) Heatmap of
transcription-regulating genes with expression that is significantly enriched
in either the LCM ZC or LCM parietal cell list.
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