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Figure 5


Fig. 5. ZCs in Mist1-/- mice abutting the neck zone (TZ2 cells) form normal serous granules but basal ZCs have fewer, smaller granules. (A) TEM of lower neck zone and upper base of a Mist1-/- gastric unit (neck zone to left). Notice how large, normal zymogenic granules occur only in cells immediately adjacent to the neck zone. A cell (TZ2, shown at higher magnification in the inset at left) with large, chimeric granules that are similar to those of normal ZCs but have a smaller electron-lucent mucus-like component as well (chimeric vesicle labeled SV, left arrow; normal vesicle indicated with arrow to right) occurs at the transition between the last parietal cell of the neck zone and the base. Outlined by a dashed box in A and shown at greater magnification in the right inset, adjacent to the TZ2 cell on the right is the highest ZC in the base; it has no residual mucus in its granules, but unlike a wild-type ZC, it has a mixture of large, normal zymogenic granules with occasional, abnormally small granules, and several granules appear in the process of fusing with the apical plasma membrane. In addition, in this region, there are invaginations of the apical plasma membrane, evidence of recently released granules. The remaining cells, farther away from the neck zone (i.e. to the right) are all abnormal: rather than having a pyramidal acinar cell morphology, they are cuboidal or columnar with irregular apical plasma membranes, and have scant supranuclear cytoplasm with abnormally small secretory granules. (B) Neck-to-base transition region in another Mist1-/- gastric unit from a section from another mouse. Again, note the abundant large granules in the immature ZC that is immediately adjacent to the last parietal cell of the neck zone and thus has not yet entirely entered the base. Residual mucus in the granules is not observed, but may be detectable by immunofluorescence; thus, this cell may either be a TZ2 cell or an early ZC. (C) Neck-to-base transition in a wild-type gastric unit for reference. Notice how all ZCs in this transition area (whether adjacent to neck zone or more mature) have large, abundant secretory granules. PC, last parietal cell of neck zone; ZC, immature ZC adjacent to neck zone.





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