DEV02722 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. ES cell lines derived fromEcad ^b+/b/b+, Ecad^+/Ncad-GFP and Ecad^{Ncad-GFP/Ncad-GFP} embryos. DIC pictures of representative ES cell colonies show normal ES cell-like morphology of wt, heterozygous and homozygous k.i. embryos.

• Supplemental Figure 2 -

  Fig. S2. Homozygous Ncad-GFP teratomas can form differentiated epithelia and express differentiation markers. Histological staining of homozygous Ncad-GFP teratomas shows formation of respiratory epithelium (A), secretory epithelium with goblet cells (B), squamous (C) and h-horn cyst-squamous epithelia (D). Monoclonal antibodies against the neuronal markers GFAP and neurofilaments (NF) show neuronal differentiation in heterozygous (E,F) and homozygous (E′,F′) teratomas. Muscle differentiation is revealed by staining with monoclonal anti-myogenin antibodies (G,G′). Scale bar: 100 μm.

• Movie 1
• Movie 2
• Movie 3
• Movie 4 -

  Movies 1-4. Time-lapse analysis of preimplantation development of Ncad k.i. embryos. The development from an 8-cell embryo to compacted morula. Homozygous mutant embryos (Movie 1) develop similarly to wt embryos (Movie 2) and compact successfully around E3.0 in vitro. Development of embryos from compacted morula to blastocyst stage. Clear defects in blastocyst formation are obvious in Ecad ^{Ncad-GFP/Ncad-GFP} embryos (Movie 3) in comparison to wt embryos (Movie 4).