|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Expression of trophectodermal and lineage specification markers in
Ncad k.i. mutants. (A,A') Double immunolabeling of
E3.5 embryos with antibodies against Oct4 (green) and Cdx2 (red) shows
predominantly ICM localization of Oct4 and trophectodermal expression of Cdx2
in wt (A) and in mutant embryos (A'). Some outer cells are also
Oct4-positive in mutant embryos (A'). (B,B')
Monoclonal TROMA-1 antibody detects intermediate filament marker cytokeratin 8
in mutant (B') and wt (B) embryos. (C,C')
Immunolocalization of ZO-1. In wt embryos ZO-1 localization is restricted to
tight junctions (C). More extended and less punctate membrane localization of
ZO-1 protein is seen in homozygous EcadNcad-GFP/Ncad-GFP
mutants (C'). (D,D') Polyclonal anti-ezrin
antibodies were used to detect microvilli on the apical pole of the outer
cells in compacted wt (D) and mutant (D') embryos.
(E,E') Actin cytoskeleton was detected with
Alexa-488-conjugated Phalloidin. Scale bar: 25 µm.
|
