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Fig. 4. Ncad k.i. embryos can attach and form blastocyst outgrowth.
DIC images of wt (A) and EcadNcad-GFP/Ncad-GFP
(A') blastocyst outgrowths. Homozygous mutant embryos were able
to attach and differentiate into trophoblast giant cells (arrow) and ICM
(arrowhead). E-cad is expressed in the ICM and downregulated in giant cells of
wt embryos (B) and is absent in the ICM of homozygous mutant embryos
(B'). The trophoblast marker antibody TROMA-1 stained cells of
both wt and mutant outgrowths (C-D'). D,D' are enlarged
pictures of the outlined areas in C,C', respectively. Scale bar: 100
µm.
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