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Fig. 6. EcadNcad-GFP/Ncad-GFP ES cells can contribute to
forming chimeric embryos. (A-C) Representative X-gal stained embryos
obtained from injection of Ecad+/Ncad-GFP ES cells into
ROSA26 (ROSA26;Ecad+/+) blastocysts that constitutively
express lacZ. Chimeric embryos with contribution of ES cells (17 of
33 analyzed embryos showed chimerism with average ES cell contribution of
50-90%) were identified by unstained cells. (A) E8.5 chimeric embryo with
approximately 50% ES cell contribution. Ecad+/Ncad-GFP ES
cells can contribute to all germ layers as seen in sections at E8.5 (B). (C)
E9.5 chimeric embryo with 90% ES cell-derived cells. (D-F) X-gal
staining of embryos derived from injections of
EcadNcad-GFP/Ncad-GFP ES cells into ROSA26 blastocysts. In
15 of 52 analyzed embryos, ES cell-derived cells are detectable, with an
average contribution to the host embryo of 5-20%. Representative embryos at
E8.5 with even distribution (D) and with contribution mainly to the tail at
E9.5 (F, arrowhead) are shown. (E) Sections of the embryo in D revealed
greater than 20% ES cell contribution to all germ layers (arrow, endoderm;
black arrowhead, mesoderm; white arrowhead, ectoderm). Scale bars: A,C,F, 500
µm; B,D,E, 100 µm.
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