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Figure 2


Fig. 2. ptpmeg is required for mushroom body (MB) axon patterning. (A) Diagram of the mushroom body (MB) with the neurons labeled in bold type. The locations of {alpha}, {alpha}', ß, and ß' lobes are indicated. (B,C) MB neurons visualized with OK107-Gal4;UAS-mCD8:GFP. In normal adults (B), dorsally projecting {alpha} and {alpha}' lobes are of similar thickness as medially projecting ß and ß' lobes. ß and ß' lobes project towards the midline but do not touch or cross it. In ptpmeg mutants (C), {alpha} and {alpha}' lobes are reduced, appearing thin and/or short (arrow), and ß and ß' lobes overgrow, touching the midline or fusing with the contralateral MB (bracket). (D-I) {alpha} and ß lobe axons from adult left brain hemispheres labeled with anti-Fasciclin II. E-G highlight the {alpha} lobe defects of ptpmeg mutants, including short (E, arrow), thin (F, arrow), and missing (G, asterisk) lobes. (H) {alpha} lobe reduction in ptpmeg mutants is rescued by expression of Ptpmeg in neurons using Elav-Gal4. (I) Animals with large clones of homozygous ptpmeg1 mutant MB tissue (marked in green) in otherwise heterozygous animals do not exhibit MB defects. (J) Normal spacing of ß lobe termini near midline. (K,L) ß lobes touch midline (K) or fuse with contralateral ß lobe (L) in ptpmeg mutants. (M) ß lobe defects in ptpmeg mutants are rescued by expression of wild-type Ptpmeg in neurons using Elav-Gal4. The midline is marked with arrowhead in J-M.





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