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Figure 6


Fig. 6. Ptpmeg protein expression in the developing and adult brain. (A) Ptpmeg expression in wild-type brain at 72 h PPF. (B) Ptpmeg expression is absent in ptpmeg1/Df(3L)ED201. (C-E) 48h PPF brain. Ptpmeg is not detectably expressed on mushroom body axons. (C) {alpha} and ß lobe axons and ellipsoid body ring axons are labeled with anti-FasII (green). Ptpmeg expression is magenta. (D-E) Ptpmeg expression (magenta), with outline of {alpha} and ß lobes and ellipsoid body superimposed in panel D. Ptpmeg is strongly expressed on ellipsoid body neurites (yellow arrowheads). (F-H) Elav-GAL4;UAS-mCD8:GFP brain at ~42 hours PPF. (F) mCD8:GFP; (G) Ptpmeg expression; (H) merge. Ptpmeg (magenta) is present on neurites, indicated by overlap with mCD8:GFP (green). Ptpmeg is also expressed in mCD8:GFP-negative regions. (I-K) Ptpmeg expression on ellipsoid body neuron cell bodies (cb) and neurites (arrowheads in K). Ptpmeg expression extends to dendritic region (ltr), but not the ellipsoid body ring. (I) Ptpmeg expression in adult. (J) Ellipsoid body neurons visualized using EB1-Gal4;UAS-mCD8:GFP. (K) Merge. (L-N) Ptpmeg expression in EB1-Gal4;UAS-mCD8:GFP;UAS-ptpmeg animal overexpressing Ptpmeg in the ellipsoid body. (L) Ptpmeg expression. (M) Ellipsoid body neurons visualized using EB1-Gal4;UAS-mCD8:GFP. (N) Merge. Note overexpressed Ptpmeg outlines cell bodies, appearing to accumulate close to the surface of the cell.





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