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Figure 2


Fig. 2. Ectopic expression of a FLAG-tagged Nanos2 protein driven by the Oct4{Delta}PE enhancer can rescue the defects in Nanos2-null testes. (A) Western blot analysis of Nanos2 from both wild-type and transgenic mouse lines expressing a FLAG-tagged Nanos2 under the control of the oct4{Delta}PE enhancer. Extracts were prepared from E14.5 male gonads and actin was used as the loading control. The level of endogenous Nanos2 was decreased in the transgenic mice. The transgene construct is schematically depicted. (B) Comparison of testis size among littermates of Nanos2+/-, Nanos2-/- and Nanos2-/- mice expressing the FLAG-tagged Nanos2 transgene background (line #5). (C-E) Sections were prepared from the testes shown in B and stained with hematoxylin and Eosin. The genotypes are indicated in each panel. (E) Some empty (asterisk) or smaller tubules were observed in the rescued testis. (F) Comparison of testis weights among different genotypes at 8 weeks. The testis weight of Nanos2+/- was designated as 100%. Scale bar: 100 µm in D for C-E.





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