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Figure 4


Fig. 4. Nanos2 can partially rescue the Nanos3-/- defects in the embryonic gonad. (A-D) Detection of migrating PGCs by ALP staining in E11.5 gonads from littermates of the Nanos3+/+ (A), Nanos3-/- (B) and Nanos3-/- expressing the Nanos2 transgene (C,D) backgrounds. (E-G) Sections were prepared from the male gonads of E14.5 Nanos3+/+ (E), Nanos3-/- (F) and Nanos3-/- expressing the Nanos2 transgene (G) backgrounds. Each section was then stained with TRA104 antibody. (H-J) Sections were prepared from the gonads of E16.5 females of Nanos3+/- (H), Nanos3-/- (I) and Nanos3-/- expressing the Nanos2 transgene (J) and stained with anti-TRA98 antibody. Signals are observed in the nuclei of germ cells. Blue, DAPI staining. Scale bars: 100 µm in E for E-G; and 100 µm in H for H-J.





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