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Figure 5


Fig. 5. Nanos3 cannot rescue the Nanos2-/- defects. (A) Western blot analysis of Nanos3 from Nanos2+/- and from Nanos2+/- mouse lines with or without a FLAG-tagged Nanos3 transgene under the control of the Nanos2 enhancer. Extracts were prepared from E14.5 male gonads. The transgene construct is schematically depicted. (B-G) Immunostaining and detection of germ cells with TRA104 antibody. Sections were prepared from E16.5 gonads (B-D) and testes at 4 weeks after birth (E-G) from Nanos2+/- (B,E), Nanos2-/- (C,F) and Nanos2-/- expressing the FLAG-tagged Nanos3 transgene (D,G) male littermates. Scale bars: 100 µm in C for B-D; and 100 µm in F for E-G.





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