DEV02622 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. That Prx1CreERT2; Tbx5^lox/lox mutant limbs are narrower than wild-type limbs is, at least in part, due to reduced cell division and not to increased apoptosis. (A) The distance between the anterior and posterior extremes of E10.5 Prx1CreERT2; Tbx5^lox/lox (n=10) limbs was shorter than for their wild-type (wt; n=12) counterparts (see main text for details). A reduction in phospho-histone H3 was observed in mutant limbs (B,C). However, no detectable difference in TUNEL staining was observed between E10.5 wild-type and Tbx5-mutant limbs (D,E).