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Figure 3


Fig. 3. Following TM administration at E8.5, the limbs of Prx1CreERT2;Tbx5lox/lox embryos are normally patterned at E10.5. Tbx5 heterozygous (control) (A,C,E,G,I,K,M,O) and mutant (B,D,F,H,J,L,N,P) embryos from pregnant females gavaged with TM at E8.5, were harvested at E10.5 and analysed by in situ hybridisation for: Tbx5 ex3 (A,B); Fgf10 (C,D); Fgf8 (E,F); Sall4 (G,H); Tbx3 (I,J); Shh (K,L); Tbx15 (M,N); Fgf8 and Myod (O,P). (B,H) Arrows indicate the heart and the anterior domain of the limb, respectively. (O, P) Somites are identified based on Myod expression and are numbered in a rostral to caudal direction.





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