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Fig. 3. SEMA3A is not required for vasculogenesis or angiogenesis. Visualisation of the cardiovasculature in stage-matched littermate mouse embryos expressing (A,C,E,G) or lacking (B,D,F,H) SEMA3A in a CD1 background. (A,B) At 9.5 dpc, endomucin-positive vessel networks have extended throughout the embryo and have begun to remodel into the large head vessels. The anterior cardinal vein (arrowhead), dorsal aorta (arrow) and the third and fourth aortic arch arteries are also clearly visible. (C-F) Higher magnification of intersomitic vessels (isv) in the trunk region between the forelimb and hindlimb buds at 9.5 (C,D) and 10.5 (E,F) dpc. Vessel branches extend between intersomitic vessels at 10.5 dpc (wavy arrows), but are rarely seen at 9.5 dpc in this region. (G-J) Double labelling of PECAM-positive vessels and neurofilament-positive nerves at 10.5 dpc reveals axon defasciculation of cranial nerves VII, IX and X (compare I and J), but no vessel defects (compare G and H) in embryos lacking SEMA3A. The heart ventricles (v), atria (a) and fourth and sixth aortic arch arteries are clearly visible in embryos lacking SEMA3A. Scale bar: 250 µm in C,D; 500 µm in A,B,E-J.

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