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Fig. 6. SEMA3A does not affect vascular patterning in the absence of
heparin/neuropilin-binding VEGF isoforms. (A-D) The
cardiovasculature of 9.5 dpc littermate mouse embryos derived from matings
between Sema3a+/- Vegfa+/120
double-heterozygous mice was labelled with antibodies specific for endomucin
(blood vessels, green) and smooth muscle actin (heart, red). The paired dorsal
aorta and anterior cardinal vein, as well as the pharyngeal arch arteries and
heart, were present in all four genotypes. (E-G) Higher magnification
of the region containing the dorsal aorta (arrowhead) and anterior cardinal
vein (arrow) shows the presence of both these large vessels in littermate
wild-type (E), Vegfa120/120 (F) and
Sema3a-/- Vegfa120/120 (G) embryos. Note that
the strong smooth muscle actin staining of the heart necessitated the use of
scanning parameters that did not record staining of the dorsal aorta, even
though it was immunoreactive. Scale bars: 500 µm.
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