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Figure 9


Fig. 9. Loss of VEGF164 but not SEMA3A impairs microvessel branching in the mouse limb. Forelimb vasculature at 12.5 dpc in stage-matched wild-type limbs (Sema3a+/+; A,F), limbs lacking SEMA3A (B,G), VEGF164 (C,H), SEMA3A and VEGF164 (D,I) or NRP1 (E,J). Microvessel networks appear normal in the absence of SEMA3A, whereas loss of VEGF164 or NRP1 impairs vessel branching. Vascular defects in limbs lacking both SEMA3A and VEGF164 are similar to those in limbs lacking only VEGF164. Scale bars: 100 µm.





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