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Figure 6


Fig. 6. Drosophila Nab physically interacts with Rn and Sqz via their conserved C-terminal domains. (A) Results of the GST-Nab pull-down assays. GST-Nab pulls down intact [35S]Rn and [35S]Sqz proteins, indicating direct binding. Binding was greatly reduced when the Rn protein lacked its C-terminal domain ([35S]Rn{Delta}854). Each experiment was repeated three times with identical results. (B) Comparison of Rn and Sqz proteins showing the two conserved domains: the zinc-finger domain (black box) with 90% homology, and the C-terminal domain (gray box). (C) Amino acid alignment of the conserved C-terminal domains of Rn and Sqz. *, identical; (:) conserved substitution; (.) semi-conserved substitution. (D) Clones of rn{Delta}854 misexpression (green, Act5C>Gal4>UASGFP UASrn{Delta}854). wg expression (red) is activated throughout in the wing pouch (arrows). (E) Clones of rn misexpression (green, Act5C>Gal4>UASGFP UASrn) only activate wg expression (red) in the wing hinge (arrows) where Nab is not expressed, but not in the wing pouch (arrowheads).





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