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Fig. 1. Effect of GDNF on GS cell proliferation. (A) Effect of GDNF
on cell recovery after 6 days in culture on MEFs (left) or laminin (right)
(mean±s.e.m.; n=5). (B) Appearance of wild-type (left
and center) and myr-Akt-Mer (right) GS cells, 6 days after passage on laminin.
(C) Apoptosis of GS cells in the absence of GDNF. Apoptotic cells were
detected by TMR red staining. Apoptotic cells were observed when GDNF was
removed from the culture medium. (D) Analysis of cell cycle
distribution, 5 days after passage on laminin. Significantly more cells are in
the S phase in the presence of GDNF or 4OHT. (E) Growth curve for
myr-Akt-Mer GS cells that were maintained by 4OHT on laminin. (F)
Effect of EGF and bFGF on cell recovery after 5 days of culture on laminin
(mean±s.e.m.; n=5). Whereas the wild-type GS cells can grow in
the presence of EGF, myr-Akt-Mer GS cells are unable to respond to EGF. Scale
bars: in B,C, 100 µm.
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