QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. Inhibition of GDNF-induced proliferation by LY294002. Cells were cultured in the presence of 33 µM LY294002 or 25 µM PD98059. (A) LY294002 decreases cell recovery after 6 days in culture on MEFs (mean±s.e.m.; n=5). (B) Analysis of cell cycle distributions, 5 days after passage on laminin in the presence of 33 µM LY294002 (left) or 25 µM PD98059 (right). Significantly more of the cells are in the G1 phase in the presence of LY294002. (C) Appearance of GS cells that were cultured on MEFs in the presence of 33 µM LY294002. Whereas control untreated cells form large colonies within 6 days (left), only small colonies are found in the presence of LY294002 (middle). However, these small colonies reinitiate growth after removal of LY294002 and form large colonies, 4 days after supplementation with fresh medium (right). (D) Quantification of spermatogonial stem cells in culture by germ cell transplantation. The same number of GS cells (2.5x10⁵ cells per six-well plate) was cultured on MEFs for 6 days with or without 33 µM LY294002, and the total number of spermatogonial stem cells was quantified by transplanting 5x10⁴ cells into the seminiferous tubules of infertile recipient testes. The total numbers of stem cells were estimated by multiplying the total cell recovery by the stem cell concentration in the injected cell suspension. Results from three independent experiments (mean±s.e.m.; n=9 for control, and n=13 for LY294002). Scale bar: in C, 100 µm.

Return to article