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Fig. 4. Phenotypic analysis of GS cells. (A) Characterization of cell
surface antigens by flow cytometry. Red shading, specific antibody; unshaded,
unstained control. (B) RT-PCR analysis of GS cells. GS cells on laminin
were cultured for 9 days in the indicated conditions. (C) Suppression
of Neurog3 transcript expression by GDNF/4OHT identified by real-time
PCR analysis (mean±s.e.m.; n=3). Expression of
Neurog3 was enhanced in both wild-type and myr-Akt-Mer GS cells in
the absence of GDNF.
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