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Fig. 1. Pygo1 and Pygo2 mutant alleles and expression of Pygo2
in the early mouse eye. (A,C) Gene targeting for
Pygo1 and Pygo2 alleles. Green and gray boxes represent
coding and non-coding exons, respectively. Light orange box indicates positive
selection marker Pgk-Neo. Dark orange bars are frt sites.
Blue arrowheads denote loxP sites. The left and right targeting arms
(LTA and RTA), N box, PHD domain and nuclear localization signal (NLS) are
indicated. Exons are numbered. Black arrows indicate the location of
amplification primers used in B,D. (B,D) PCR amplification of
genomic DNA or cDNA (the lower panel in D) for Pygo1 (B) or
Pygo2 (D) allelic series. (E-J) Immunofluorescence for Pygo2
(E,F,H,I) or Pax6 (G,J) in eye region cryosections from the embryonic stages
and genotypes indicated. A gray line between panels indicates that different
channels of the same image are displayed. ple, presumptive lens ectoderm; op,
optic pit; lp, lens placode; ov, optic vesicle.
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