spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Pygo2 is required for lens development in the mouse. (A-C) Hematoxylin and Eosin-stained sections of eyes in wild-type (WT) and Pygo2-/- mice show mildly (B, small lens) and severely (C, no lens) affected mutants. (D-F) GFP fluorescence in whole-mount embryos again showing small (E) or absent (F) lenses in the mutant. (G-I) Eye region cryosections labeled for the lens marker ß-crystallin (green) and for nuclei with Hoechst 33258 (blue). A small group of ß-crystallin-positive cells is indicated by the arrow in I. (J,L) Whole-mount embryos showing the WT and gross Pygo2-/- phenotype. The arrow in L indicates pigmented tissue within the eye cup. (K) Box plot of the lens:optic cup diameter ratio in allelic series of Pygo1 and Pygo2 mutants. Each bar represents measurements from ten eyes in five embryos. One-way ANOVA indicated statistically significant changes as compared with the wild-type control (**, P≤0.01≥0). ret, retina; mes, mesenchyme; pce, presumptive conjunctival epithelium; pr, presumptive retina.





Right arrow Return to article