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Fig. 3. Pygo2 regulates Pax6 expression in the mouse lens placode.
(A-P) Eye region cryosections labeled for nuclei with Hoechst 33258
(A-N, blue), Pax6 (A-F, red), GFP (G,H, green), Sox2 (I,J, red), Pygo2 (K-N,
red) or unlabeled differential interference contrast (DIC) images (O,P). The
arrowhead in N (lower panel) indicates a small group of Pygo2-labeled cells in
the presumptive lens. ple, presumptive lens ectoderm; ov, optic vesicle; lp,
lens placode; pi, lens pit; pr, presumptive retina. A gray line between panels
indicates that different channels of the same image are displayed. (Q)
Box plot showing the ratio of lens to optic cup diameter in E12.5 embryos of
the indicated genotypes. Mutant values (green and red bars) were significantly
different (**, P
0.01) from the wild-type and
Le-cre controls (yellow bars). In addition, the Le-cre
conditional mutant (green bar) is significantly different (**,
P0.01) from the germline null (red bar). The wild-type control
value incorporates the ten samples from
Fig. 2K and a new set of 12
control values from this experiment which yielded the Le-cre
conditional mutants. Germline null values are reproduced from
Fig. 2K for the purposes of
comparison.
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