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Fig. 1. Transient Olig2 expression in developing cortical astrocytes.
(A-E) Sagittal cortical sections from wild-type mice at P5 were
immunostained with antibodies against GFAP, Olig2 or glutamine synthetase
(GS). (A) GFAP+ cells (red) at this stage expressed a high level of
Olig2 (green) as judged by staining intensity. Orthogonal reconstructions of
confocal images at z-axis level are shown in side panels (along the
right-hand edge and beneath). Arrowheads indicate GFAP+
Olig2+ double-positive cells. A GFAP+ Olig2+
cell, marked by cross-lines, is shown at a higher magnification in B-B''.
(C-E) Co-expression (E) of GFAP (C, red) and GS (D, green) was observed in the
astrocytes of the neonatal cortex. (F-K) Sagittal cortical sections of
wild-type mice at P5 (F,G), P14 (H,I) and P21 (J,K) were immunostained with
antibodies against Olig2 and GS. Confocal images at z-axis level are
shown in side panels of G and I. (F,G) GS+ astrocytes (red) express
a high level of Olig2 (green) at P5. Arrowheads indicate GS+
Olig2+ double-positive cells. (H,I) At P14, Olig2 expression in
GS+ astrocytes (arrowheads) is reduced to a low level as compared
with the intense Olig2 expression in GS- cells (blue arrows). (J,K)
At P21, Olig2 expression (green) is undetectable in GS+ astrocytes
(red) in the cortex. (L-O) The corpus callosum region of wild-type mice
at P5 was immunostained with antibodies against GFAP and Olig2. Arrowheads
indicate GFAP+ Olig2+ double-positive cells in this
developing white matter. The boxed area in L is shown at a higher
magnification in M-O. Orthogonal reconstructions of confocal images at
z-axis level are shown for a GFAP+ Olig2+ cell
marked by crosslines in O. Scale bars: 50 µm in A,F-L.
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