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Fig. 5. White matter astrocyte formation deficiency in the Olig2 mutant
spinal cord. In situ hybridization and immunostaining of Olig2, GFAP,
S100ß and GS were performed on frozen spinal sections of
CtrlG and CkoG mice at P14. Arrows
indicate the white matter region of the spinal cord. (A,B) mRNA
expression of Gfap was examined in the control (A) and Olig2
(B) mutant spinal cord by in situ hybridization. (C-F) Expression of
Olig2 and GFAP was analyzed by immunohistochemistry in the control (C) and
Olig2 mutant (E) spinal cord. GFAP expression in C,E is shown in D,F,
respectively. GFAP is strongly expressed in the spinal white matter of the
control, but only weakly in the mutant (arrows). (G-N) Expression of
Olig2 and S100ß (G,I), and Olig2 and GS (K,M), were analyzed by
immunohistochemistry in the spinal cord of control (G,H,K,L) and
Olig2 mutant (I,J,M,N) mice. Staining of S100ß and GS is shown
in H,J and L,N, respectively. S100ß- and GS-expressing cells are barely
detectible in the white matter of Olig2 mutants (J,N) as compared
with controls (H,L). (O) Bar chart showing the average number of
S100ß+ and GS+ cells per unit area (0.06
mm2) in the spinal white matter of control and Olig2
mutant mice (n=3); bars indicate s.d. Scale bar: 100 µm in
A-N.
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