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Fig. 7. Mutagenesis of AP1-binding site causes ectopic expression of
AGL24 in young floral meristems. (A) Schematic of the
ProAGL24:GUS construct where the 4.7 kb Arabidopsis
AGL24 genomic sequence was translationally fused with the GUS gene. The
native CArG box near the AGL24-4 fragment identified in
Fig. 6E was mutated.
(B,C) GUS staining in inflorescence apices of the transformants
containing ProAGL24:GUS (B) and its derived construct with
the mutated CArG box (C). At least 12 independent lines for each construct
were analyzed and representative images are shown. Scale bars: 100 µm for
B,C.