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Figure 3


Fig. 3. Deficit of KA' interneurons is independent of apoptosis in mib-/- zebrafish embryos. Dorsal views of the posterior neural plate at 14 hpf (A,C) and lateral views of spinal cord at 25 hpf (B,D) in Tg(olig2:egfp) (A,B) and mib-/-;Tg(olig2:egfp) embryos (C,D). Green, blue and red channels mark EGFP+ cells, Isl+ neurons and TUNEL+ cells, respectively. Neither control nor mib-/- embryos had EGFP+ TUNEL+ cells at 14 hpf or 25 hpf (A-D). TUNEL+ Rohon-Beard neurons (arrowheads) were detected in control and mib-/- embryos (B,D) at 25 hpf. Scale bar: 25 µm.





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