QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 5. Conditional activation of Notch signaling produces excess KA' interneurons at the expense of PMNs. (A-F) Lateral views of the spinal cord of zebrafish embryos at the 6- to 12-somite region, with anterior to the left and dorsal at the top. (A,B) Control Tg(UAS:Notch1a^ac-myc);Tg(olig2:egfp), (C,D) Tg(hsp70l:gal4vp16);Tg(UAS:Notch1a^ac-myc);Tg(olig2:egfp) and (E,F) Tg(hsp70l:XdnSu(H)myc);Tg(olig2:egfp) embryos were heat shocked at 11 hpf. (A,B) Control embryos showed normal numbers of isl2⁺ PMNs (black bracket), dorsal Rohon-Beard sensory neurons (white bracket) and GABAergic KA' cells (arrowheads). (C) isl2⁺ PMNs (black bracket) and Rohon-Beard neurons (white bracket) were almost absent from heat-shocked triple-transgenic embryos. (D) Heat-shocked triple-transgenic embryos had excess KA' interneurons (arrowheads). (E,F) Heat-shocked Tg(hsp70l:XdnSu(H)myc);Tg(olig2:egfp) embryos had excess PMNs (black bracket), excess Rohon-Beard neurons (white bracket) and a deficit of KA' interneurons (arrowheads). (G) Mean of the number of PMNs (gray bar) and KA' interneurons (black bar) per hemisegment (n=7) in A-F. Error bars represent s.e.m. Scale bar: 50 µm.

Return to article