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Figure 6


Fig. 6. Individual olig2:EGFP+ neural plate cells produce single types of neurons in mib-/- embryos. (A-E) Lateral views of the spinal cord of 24- to 30-hpf mib-/-;Tg(olig2:egfp) zebrafish embryos, with anterior to left and dorsal at the top. In each case, a single EGFP+ cell was labeled with vital dye (red) at 10 hpf. (A,B) Labeled cells that did not divide but differentiated into a PMN and VeLD. (C,D) Examples of individual EGFP+ clones that produced only PMNs, as determined by axons that projected out of the spinal cord to muscle. (E) Example of an EGFP+ clone that produced two VeLD interneurons. Scale bar: 20 µm.





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