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Fig. 1. Mutations in AtSWC6 and SUF3 cause the same
phenotype. (A) Genomic structure of AtSWC6 and T-DNA
location in the atswc6 mutant (SAIL_1142/CS841940). Black and gray
boxes indicate exons and untranslated regions, respectively; lines between
these boxes indicate introns; white triangle indicates T-DNA insertion;
arrowhead indicates transcription start site. (B) RT-PCR analysis of
AtSWC6, FLC and SUF3 expression in wild type and
atswc6 mutant. RNA was extracted from 30-day-old Arabidopsis
plants grown in long-day conditions. TUBULIN (TUB) was used
as an internal control. (C) Northern blot analysis of AtSWC6
and FLC expression. RNA was extracted from 10-day-old FRI, atswc6
FRI, suf3 FRI and Col. The AtSWC6 transcript was not detected in
the atswc6 mutant, indicating that it is a null allele. (D)
Flowering time of FRI, atswc6 FRI and suf3 FRI and of Col
(fri), atswc6 fri and suf3 fri grown under long-day
conditions. (E) Morphology of wild type (Col) and atswc6 grown
for 20 days under long-day conditions. (F) Morphology of FRI, suf3
FRI, atswc6 FRI and suf3 atswc6 FRI grown for 30 days under
long-day conditions. (G) Leaf shape of wild type (Col) and
atswc6 mutant. (H) atswc6 flower with five petals.
Scale bars: in E and G, 1 cm.
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