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Fig. 2. Phenotype of AtSWC6 overexpression line and chromatin
immunoprecipitation. (A) Col, atswc6 and
35S-myc:AtSWC6 atswc6 transgenic Arabidopsis plants grown
for 35 days. (B) Col, atswc6 and 35S-AtSWC6:GFP
atswc6 transgenic plant grown for 35 days. (C) Number of rosette
leaves at flowering of Col, atswc6, 35S-myc:AtSWC6 atswc6 and
35S-AtSWC6:GFP atswc6. All plants in A-C were grown in long-day
conditions. (D) Expression of FT, SOC1 and FLC in
Col, suf3, atswc6, 35S-myc:AtSWC6 atswc6 and 35S-AtSWC6:GFP
atswc6 grown in short-day conditions for 4 weeks. Early flowering of
atswc6 and suf3 is caused by the increased expression of the
floral integrators FT and SOC1. (E) Col and
35S-myc:SUF3 suf3 (for SUF3 protein) or 35S-myc:AtSWC6
atswc6 (for AtSWC6 protein) transgenic seedlings grown under long-day
conditions were used for ChIP assay using anti-myc antibody. Above is a map of
the FLC gene showing the location of the regions examined in the
FLC promoter (FLC-A for -1288 to -1470, FLC-B for -360 to -497, FLC-C
for -45 to -164, relative to the ATG codon) and first intron (FLC-V for
+1469-+1607). TUB was used as an internal control (white bars). The
x-axis indicates relative enrichment.
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